PROLONGED BIOCHEMICAL AND MORPHOLOGICAL STABILITY OF ENCAPSULATED LIVER-CELLS - A NEW METHOD

被引：25

作者：

STANGE, J

MITZNER, S

DAUTZENBERG, H

RAMLOW, W

KNIPPEL, M

STEINER, M

ERNST, B

SCHMIDT, R

KLINKMANN, H

机构：

[1] INST POLYMER CHEM,TELTOW,GERMANY

[2] UNIV ROSTOCK,DEPT CLIN CHEM,O-2500 ROSTOCK,GERMANY

来源：

BIOMATERIALS ARTIFICIAL CELLS AND IMMOBILIZATION BIOTECHNOLOGY | 1993年 / 21卷 / 03期

关键词：

D O I：

10.3109/10731199309117372

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this work a new type of polyelectrolyte complex capsules is introduced as an artificial housing for liver cells. Male Wistar rat hepatocytes were encapsulated using cellulose sulphate and polydimethyldiallylammonium chlorid as polyelectrolytes. Amino acid metabolism rate and urea synthesis of the cells increased over the investigation period in contrast to the decrease observed in control monolayer cultures. The encapsulated cells were morphologically characterized. The described procedure represents a sufficient method for the cultivation of living cells in mechanically stable semipermeable microcapsules.

引用

页码：343 / 352

页数：10

共 11 条

[1]

BRUGMANN E, 1990, BIOMAT ART CELLS ART, V18, P51

[2]

CHANG TMS, 1987, BIOMATER ARTIF CELL, V15, P1

[3] HEPATOCYTE PROLIFERATION INVITRO - ITS DEPENDENCE ON THE USE OF SERUM-FREE HORMONALLY DEFINED MEDIUM AND SUBSTRATA OF EXTRACELLULAR-MATRIX [J].