INTERACTION OF CIBACRON BLUE F3GA WITH ESCHERICHIA-COLI DNA-POLYMERASE-I AND WITH T4 DNA-POLYMERASE

被引：10

作者：

LINDELL, TD

NICHOLS, BP

DONELSON, JE

STELLWAGEN, E

机构：

[1] Department of Biochemistry, University of Iowa, Iowa City

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 562卷 / 02期

基金：

美国国家科学基金会;

关键词：

(Purification; T4; phage; Escherichia coli); Blue dextran-Sepharose; Cibacron Blue; DNA polymerase;

D O I：

10.1016/0005-2787(79)90169-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Individual rapid procedures for the enrichment of Escherichia coli DNA polymerase I and of bacteriophage T4 DNA polymerase free of endonuclease activity are described using Blue dextran-Sepharose chromatography. The blue dye of Blue dextran-Sepharose selectively binds to the deoxynucleoside triphosphate substrate site of the E. coli but not the T4 enzyme indicating that the catalytic sites of these two enzymes which catalyze the same polymerization reaction in vitro are quite distinct. © 1979.

引用

页码：231 / 239

页数：9

共 13 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] BLUE-DEXTRAN-SEPHAROSE AFFINITY CHROMATOGRAPHY - RECOGNITION OF A POLYNUCLEOTIDE BINDING-SITE OF A PROTEIN [J].