TRANSIENT INVIVO PROTEIN ADSORPTION ONTO POLYMERIC BIOMATERIALS

被引:40
作者
IHLENFELD, JV [1 ]
COOPER, SL [1 ]
机构
[1] UNIV WISCONSIN,DEPT CHEM ENGN,MADISON,WI 53706
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 1979年 / 13卷 / 04期
关键词
D O I
10.1002/jbm.820130405
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The adsorption of albumin, γ‐globulin, and fibrinogen was measured on three ex vivo polymeric shunt surfaces [polyvinyl chloride (PVC), Silastic, and segmented polyether urethane (Biomer)] exposed to flowing heparinized, canine blood in vivo. Small amounts of radiolabeled proteins were infused into anesthetized mongrel dogs and the deposition of radioactivity on the walls of femoral arteriovenous shunts was followed with time for two hours following initial blood‐polymer contact. Previously, transient in vivo platelet and fibrin deposition onto PVC, Silastic, and Biomer was measured by a similar technique in the absence of anticoagulant. A time‐dependent phase of thrombus deposition followed by thromboembolism was observed on the PVC and Silasticshunt surfaces but not on the Biomer surface. In the studies reported here on PVC and Silastic, fibrinogen adsorption was found to predominate initially, though it subsequently desorbed somewhat and was replaced by albumin and γ‐globulin. On Biomer, the adsorption of all three proteins increased with time following initial blood contact and fibrinogen was less prominent initially. The PVC surface was found to become passivated with respect to further thrombogenesis after 60‐min exposure to flowing blood, at which time a higher fraction of albumin was present on the surface compared to that at earlier blood contact times. These results indicate that rearrangement of adsorbed protein species occurs with time on polymer surfaces exposed to flowing blood in vivo. Early and predominant fibrinogen adsorption appears to be an important factor in the thrombogenic and embolic events observed on the PVC and Silastic shunt surfaces in vivo. Copyright © 1979 John Wiley & Sons, Inc.
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页码:577 / 591
页数:15
相关论文
共 23 条
[1]  
Baier R E, 1969, J Biomed Mater Res, V3, P191, DOI 10.1002/jbm.820030115
[2]  
BAIER RE, 1971, FED PROC, V30, P1523
[3]  
BARBER TA, 1978, SCANNING ELECTRON MI, V2, P431
[4]  
Brash J L, 1969, J Biomed Mater Res, V3, P175, DOI 10.1002/jbm.820030114
[5]  
BRASH JL, 1974, T AM SOC ART INT ORG, VA 20, P69
[6]  
BRASH JL, 1976, T AM SOC ART INT ORG, V22, P253
[7]  
Dutton R C, 1969, J Biomed Mater Res, V3, P13, DOI 10.1002/jbm.820030104
[8]  
HORBETT TA, 1975, ADV CHEM SER, V145, P230
[9]  
IHLENFELD JV, 1978, T AM SOC ART INT ORG, V24, P727
[10]  
IHLENFELD JV, 1978, THESIS U WISCONSIN