IDENTIFICATION OF A PEPTIDE SPECIFIC FOR APLYSIA SENSORY NEURONS BY PCR-BASED DIFFERENTIAL SCREENING

被引：81

作者：

BRUNET, JF ^{[1
]}

SHAPIRO, E ^{[1
]}

FOSTER, SA ^{[1
]}

KANDEL, ER ^{[1
]}

IINO, Y ^{[1
]}

机构：

[1] COLUMBIA UNIV COLL PHYS & SURG,CTR NEUROBIOL & BEHAV,NEW YORK,NY 10032

来源：

SCIENCE | 1991年 / 252卷 / 5007期

关键词：

D O I：

10.1126/science.1840700

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In order to identify genes specific for the sensory neurons of Aplysia, a miniaturized differential screening method based on the polymerase chain reaction and applicable to small amounts of tissue was used. One messenger RNA was isolated that is expressed in every mechanoreceptor sensory cluster of the Aplysia central nervous system. This messenger RNA encodes a peptide that seems to function as an inhibitory cotransmitter. The peptide selectively inhibits certain postsynaptic cells but not others and thereby allows the sensory neurons to achieve target-specific synaptic actions.

引用

页码：856 / 859

页数：4

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