IDENTIFICATION OF PRO-EPIDERMAL GROWTH-FACTOR AND HIGH-MOLECULAR-WEIGHT EPIDERMAL GROWTH-FACTORS IN ADULT-MOUSE URINE

被引：22

作者：

LAKSHMANAN, J

SALIDO, EC

LAM, R

BARAJAS, L

FISHER, DA

机构：

[1] Department of Pediatrics and Pathology, Harbor UCLA Medical Center, UCLA School of Medicine, CA 90509, Walter P. Martin Research Bldg. Torrance

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 173卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)80871-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By use of immunoblot analysis, we demonstrate the presence of a pro-Epidermal growth factor (EGF) with an approximate molecular weight of 165 kDa in adult mouse urine. In addition, urine contains four high molecular weight EGFs with approximate molecular weights of 116, 97, 66 and 56 kDa. The 165 kDa pro-EGF as well as the 66 and 56 kDa EGFs also are detectable in mouse kidney extract. Neither urine nor kidney contain the mature EGF of 6 kDa. The 165 kDa pro-EGF is the major product synthesized in renal tissue and secreted in urine. The finding of high molecular weight EGFs in urine suggests that part of pro-EGF secreted into urine undergoes partial proteolysis distal to its site of synthesis. © 1990 Academic Press, Inc.

引用

页码：902 / 911

页数：10

共 22 条

[1] MACULA DENSA CELLS OF MOUSE KIDNEY DO NOT SYNTHESIZE EPIDERMAL GROWTH-FACTOR PRECURSOR MESSENGER-RNA [J].

ATKIN, BM ;

FRANKLIN, WA ;

BELL, GI ;

TOBACK, FG .

NEPHRON, 1990, 54 (04) :313-317

[2] HUMAN EPIDERMAL GROWTH-FACTOR PRECURSOR - CDNA SEQUENCE, EXPRESSION INVITRO AND GENE ORGANIZATION [J].

BELL, GI ;

FONG, NM ;

STEMPIEN, MM ;

WORMSTED, MA ;

CAPUT, D ;

KU, L ;

URDEA, MS ;

RALL, LB ;

SANCHEZPESCADOR, R .

NUCLEIC ACIDS RESEARCH, 1986, 14 (21) :8427-8446

[3]

BREYER JA, 1990, J BIOL CHEM, V265, P16564

[4]

Carpenter G., 1990, HDB EXPT PHARM, P69

[5]

COHEN S, 1962, J BIOL CHEM, V237, P1555

[6] CLONING AND SEQUENCE-ANALYSIS OF A CDNA FOR RAT EPIDERMAL GROWTH-FACTOR [J].

DOROW, DS ;

SIMPSON, RJ .

NUCLEIC ACIDS RESEARCH, 1988, 16 (19) :9338-9338

[7]

FISHER DA, 1990, J ENDOCR REV, V11, P418

[8] NUCLEOTIDE-SEQUENCE OF EPIDERMAL GROWTH-FACTOR CDNA PREDICTS A 128,000-MOLECULAR WEIGHT PROTEIN-PRECURSOR [J].

GRAY, A ;

DULL, TJ ;

ULLRICH, A .

NATURE, 1983, 303 (5919) :722-725

[9] PROTEOGLYCAN PROFILES OBTAINED BY ELECTROPHORESIS AND TRIPLE IMMUNOBLOTTING [J].

HEIMER, R .

ANALYTICAL BIOCHEMISTRY, 1989, 180 (02) :211-215

[10]

HENRY R, 1967, CLIN CHEM PRINCIPLES, P266

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