IS THERE A UNIQUE RIBOSOME PHENOTYPE FOR NATURALLY-OCCURRING ESCHERICHIA-COLI

被引：32

作者：

MIKKOLA, R

KURLAND, CG

机构：

[1] Department of Molecular Biology, Uppsala University, Biomedical Center, Uppsala, S751 24

来源：

BIOCHIMIE | 1991年 / 73卷 / 7-8期

关键词：

NATURAL ESCHERICHIA-COLI; GROWTH PHENOTYPES; RIBOSOME KINETICS;

D O I：

10.1016/0300-9084(91)90148-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have compared the growth characteristics of natural isolates of E coli with the kinetic properties of their ribosomes in vitro. The variability of the different performance characteristics of ribosomes isolated from natural isolates of E coli show that there is not a unique wild-type ribosome phenotype just as there is not a unique growth phenotype for the bacteria. In addition, there is a strong correlation between the growth rates and the efficiency of the kinetic interaction between the ribosomes and the EF-Tu-GTP-aminoacyl-tRNA ternary complex in vitro. No such correlation is seen between the growth rate and the maximum turnover rate of the ribosomes in vitro. These data suggest that the codon-programmed ribosomes are not kinetically saturated with ternary complexes in vivo.

引用

页码：1061 / 1066

页数：6

共 19 条

[1] KINETIC IMPAIRMENT OF RESTRICTIVE STREPTOMYCIN-RESISTANT RIBOSOMES [J].