ASSIGNMENT OF THE CHICKEN TYROSINE-HYDROXYLASE GENE TO CHROMOSOME-6 BY FISH

被引：14

作者：

DOMINGUEZSTEGLICH, M

CARRIER, A

AUFFRAY, C

SCHMID, M

机构：

[1] UNIV WURZBURG,DEPT HUMAN GENET,BIOZENTRUM HUBLAND,W-8700 WURZBURG,GERMANY

[2] COLL FRANCE,INST EMBRYOL,CNRS,NOGENT SUR MARNE,FRANCE

来源：

CYTOGENETICS AND CELL GENETICS | 1992年 / 60卷 / 02期

关键词：

D O I：

10.1159/000133324

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The gene for tyrosine hydroxylase, the first and rate-limiting enzyme in the biosynthetic pathway of catecholamine neurotransmitters, has been localized in situ to chromosome 6 in the chicken. It is the first DNA marker to be reported for this telocentric macrochromosome. Use of a 45-kb biotinylated chicken-specific cosmid probe and a sensitive fluorescent detection system proved to be highly efficient, with over 90% of metaphases showing positive hybridization signals on one or (usually) both chromosome 6 homologs, in physically mapping this single-gene locus.

引用

页码：138 / &

共 28 条

[1] MODULATION OF TYROSINE-HYDROXYLASE GENE-EXPRESSION IN THE CENTRAL-NERVOUS-SYSTEM VISUALIZED BY INSITU HYBRIDIZATION [J].