HLA-DQB1 AND DQA1 MATCHING BY AMBIENT-TEMPERATURE PCR-SSCP

被引：11

作者：

CLAY, TM ^{[1
]}

CULPAN, D ^{[1
]}

PURSALL, MC ^{[1
]}

BRADLEY, BA ^{[1
]}

BIDWELL, JL ^{[1
]}

机构：

[1] UNIV BRISTOL,DEPT TRANSPLANTAT SCI,BRISTOL BS6 6JU,AVON,ENGLAND

来源：

EUROPEAN JOURNAL OF IMMUNOGENETICS | 1995年 / 22卷 / 06期

关键词：

D O I：

10.1111/j.1744-313X.1995.tb00283.x

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have developed a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) protocol for rapid matching of DQA1 and DQB1 alleles. Electrophoresis can be performed at ambient temperature within the range 18-28 degrees C without continuous gel cooling. The method has been tested on 27 patient-potential bone marrow donor pairs for DQB1 and 31 pairs for DQA1. Bone marrow pairs were chosen to represent a broad range of common alleles based upon previous restriction fragment length polymorphism (RFLP) analysis type assignments. Samples were re-typed by PCR with sequence-specific primers (PCR-SSP) and the results compared to matching by PCR-SSCP analysis. There was a 100% correlation between PCR-SSP and PCR-SSCP analysis for DQB1, and a 97% correlation for DQA1 matching.

引用

页码：467 / 478

页数：12

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