PRELIMINARY EVALUATION OF CYTO-RICH - AN IMPROVED AUTOMATED CYTOLOGY PREPARATION

被引：48

作者：

GEYER, JW

HANCOCK, F

CARRICO, C

KIRKPATRICK, M

机构：

[1] Roche Biomedical Laboratories, Burlington, North Carolina

[2] University of Nebraska Medical Center, Omaha, Nebraska

来源：

DIAGNOSTIC CYTOPATHOLOGY | 1993年 / 9卷 / 04期

关键词：

CERVICAL SMEAR; CERVICAL MONOLAYER; PAP SMEAR;

D O I：

10.1002/dc.2840090409

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A new cervical cytology monolayer preparation system called Cyto-Rich was evaluated. Using samples from 557 patients, Cyto-Rich monolayers were compared to matched conventional smears. After conventional smears were prepared and spray fixed, residual exfoliated cells were transferred to preservative fluid. The cell suspensions were gently disaggregated and the epithelial component enriched with gradient centrifugal sedimentation. The batched samples were then placed on the Cyto-Rich work station where slides are automatically prepared and stained. The results demonstrate that Cyto-Rich prepared monolayers are vastly superior to the conventional smears for cell presentation. While the study showed 99% overall concordance, Cyto-Rich improved the detection of low-grade cervical intraepithelial lesions. (C) 1993 Wiley-Liss, Inc.

引用

页码：417 / 422

页数：6

共 22 条

[1] van Driel-Kulker ANJ, Ploem-Zaaijer JJ, van der Zwan-van der Zwan M, Tanke HJ, A preparation technique for exfoliated and aspirated cells allowing different staining procedures, Analyt Quant Cytol, 2, pp. 243-246, (1980)
[2] Husain OAN, Page-Roberts BA, Millet JA, A sample preparation for automated cervical cancer screening, Acta Cytol, 22, pp. 15-21, (1978)
[3] Naujoks H, Strohmeier R, Bicker T, van Driel-Kulker AMJ, Knepfle CFHM, Ploem JS, Interobserver variability in the cytological diagnosis of 1,500 Papanicolaou stained cervical monolayer specimens, Path Res Pract, 186, pp. 150-153, (1990)
[4] Rosenthal DL, Stern E, McLatchie C, Wu A, Lagasse LD, Wall R, Castleman KR, A simple method of producing a monolayer of cervical cells for digital image processing, Anal Quan Cytol, 2, pp. 84-88, (1979)
[5] Bahr GF, Bibbo M, Oehme M, Puls JH, Reale FR, Wied GL, An automated device for the production of cell preparations suitable for automatic assessment, Acta Cytol, 22, pp. 243-249, (1978)
[6] Vooijs GP, Benign proliferative reactions, intraepithelial neoplasia and invasive cancer of the uterine cervix, Comprehensive cytopathology, pp. 153-230, (1991)
[7] Gupta PK, Microbiology, inflammation and viral infections, Comprehensive cytopathology, pp. 115-152, (1991)
[8] Rosenthal DL, Manjikian V, Techniques in the preparation of a monolayer of gynecologic cells for automated cytology, an overview, Anal Quan Cytol Histol, 9, pp. 55-59, (1987)
[9] Mead JS, Horan PK, Wheeless LL, Syringing as a method of cell dispersal. I. Effect on intermediate and superficial squamous cells, Acta Cytol, 22, pp. 86-90, (1978)
[10] Lopez PA, Cambier MA, Wheeless LL, Syringing as a method of cell dispersal. II. Effect on abnormal cells, Anal Quan Cytol, 3, pp. 235-238, (1981)

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