A U3 SMALL NUCLEAR RIBONUCLEOPROTEIN-REQUIRING PROCESSING EVENT IN THE 5' EXTERNAL TRANSCRIBED SPACER OF XENOPUS PRECURSOR RIBOSOMAL-RNA

被引：87

作者：

MOUGEY, EB ^{[1
]}

PAPE, LK ^{[1
]}

SOLLNERWEBB, B ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,725 N WOLFE ST,BALTIMORE,MD 21205

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1993年 / 13卷 / 10期

关键词：

D O I：

10.1128/MCB.13.10.5990

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A processing site has been identified within the 5' external transcribed spacer (ETS) of Xenopus laevis and X. borealis pre-RNAs, and this in vivo processing can be reproduced in vitro. It involves a stable and specific association of the pre-rRNA with factors in the cell extract, including at least four RNA-contacting polypeptides, yielding a distinct complex that sediments at 20S. Processing also requires the U3 small nuclear RNA. This processing, at residue +105 of the 713-nucleotide X. laevis 5' ETS, is highly reminiscent of the initial processing cleavage of mouse pre-rRNA within its 3.5-kb 5' ETS, previously thought to be mammal specific. The frog and mouse processing signals share a short essential sequence motif, and mouse factors can faithfully process the frog pre-rRNA. This conservation suggests that this 5' ETS processing site serves an evolutionarily selective function.

引用

页码：5990 / 5998

页数：9

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