TRANSITION-STATE ANALOGS AS AFFINITY LABELS FOR HUMAN DNA METHYLTRANSFERASES

被引：10

作者：

BAKER, DJ ^{[1
]}

LAAYOUN, A ^{[1
]}

SMITH, SS ^{[1
]}

机构：

[1] CITY HOPE NATL MED CTR,DEPT CELL & TUMOR BIOL,1500 E DUARTE RD,DUARTE,CA 91010

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.2329

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new class of affinity labels has been developed for human DNA (cytosine-5) methyltransferase. These oligodeoxynucleotides contain 5-fluorodeoxycytidine at a mispair within the recognition motif of the human enzymes. They were not effectively recognized by bacterial methyltransferases. They can be viewed as analogs of the intermediates transiently produced by methyltransferases during catalysis. Affinity labelling patterns suggest that both the structurally induced activity and the methyl-directed activity of the human enzymes operate by the same mechanism and reside on the same polypeptide chains. © 1993 Academic Press, Inc.

引用

页码：864 / 871

页数：8

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