ISOLATION OF A MESSENGER-RNA ENCODING A NUCLEOSIDE DIPHOSPHATE KINASE FROM TOMATO THAT IS UP-REGULATED BY WOUNDING

被引：45

作者：

HARRIS, N ^{[1
]}

TAYLOR, JE ^{[1
]}

ROBERTS, JA ^{[1
]}

机构：

[1] UNIV NOTTINGHAM, FAC AGR & FOOD SCI, DEPT PHYSIOL & ENVIRONM SCI, LOUGHBOROUGH LE12 5RD, LEICS, ENGLAND

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 25卷 / 04期

关键词：

LYCOPERSICON ESCULENTUM; TOMATO; NUCLEOSIDE DIPHOSPHATE KINASE; WOUNDING; ETHYLENE;

D O I：

10.1007/BF00029611

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone (TAB2) encoding a nucleoside diphosphate (NDP) kinase has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) cDNA library. The clone is 590 bp long and exhibits a high degree of sequence identity with spinach NDP kinases I and II, Pisum sativum NDP kinase I, Arabidopsis thaliana NDP kinase, Drosophila melanogaster NDP kinase, Dictyostelium discoideum NDP kinase and human Nm 23-H1 and Nm23-H2. Northern analysis has revealed that the mRNA encoded by TAB2 is up-regulated in both leaf and stem tissue in response to wounding. The increase is apparent within 1 h of wounding and is not further elevated by application of ethylene. Southern blot analysis indicates that TAB2 is a member of a small gene family.

引用

页码：739 / 742

页数：4

共 19 条

[1] ACTIVATION OF G-PROTEINS BY RECEPTOR-STIMULATED NUCLEOSIDE DIPHOSPHATE KINASE IN DICTYOSTELIUM [J].