ISOLATION AND IDENTIFICATION OF 5,6-EPOXYRETINOIC ACID - BIOLOGICALLY-ACTIVE METABOLITE OF RETINOIC ACID

被引：80

作者：

MCCORMICK, AM ^{[1
]}

NAPOLI, JL ^{[1
]}

SCHNOES, HK ^{[1
]}

DELUCA, HF ^{[1
]}

机构：

[1] UNIV WISCONSIN,COLL AGR & LIFE SCI,DEPT BIOCHEM,MADISON,WI 53706

来源：

BIOCHEMISTRY | 1978年 / 17卷 / 19期

关键词：

D O I：

10.1021/bi00612a033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly biologically active metabolite of retinoic acid (8111) has been isolated in pure form from intestinal mucosa of vitamin A deficient rats given [3H] retinoic acid. This metabolite has been positively identified as 5,6-epoxyretinoic acid based on the ultraviolet absorption spectrum and mass spectrum of its methylated derivative. This identification was confirmed by cochromatography of the methylated metabolite and synthetic methyl 5,6-epoxyretinoate on reverse-phase and straight-phase high-pressure liquid chromatography. The 5,6-epoxyretinoic acid is a true in vivo generated metabolite of retinoic acid and not an artifact of the isolation procedure. In addition, 5,8-oxyretinoic acid previously isolated in this laboratory from intestinal mucosa was probably generated from 5,6-epoxyretinoic acid by the acidic conditions used in the extraction and isolation of the 5,8-oxyretinoic acid. © 1978, American Chemical Society. All rights reserved.

引用

页码：4085 / 4090

页数：6

共 30 条

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