ENDOTHELIN-INDUCED INTRACELLULAR CA2+ MOBILIZATION THROUGH ITS SPECIFIC RECEPTORS IN MURINE PERITONEAL-MACROPHAGES

被引：35

作者：

KISHINO, J ^{[1
]}

HANASAKI, K ^{[1
]}

KATO, T ^{[1
]}

ARITA, H ^{[1
]}

机构：

[1] SHIONOGI & CO LTD,SHIONOGI RES LAB,12-4 SAGISU 5-CHOME,FUKUSHIMA KU,OSAKA 553,JAPAN

来源：

FEBS LETTERS | 1991年 / 280卷 / 01期

关键词：

ENDOTHELIN; RECEPTOR BINDING; CYTOSOLIC FREE CA2+; PERITONEAL MACROPHAGES; MOUSE;

D O I：

10.1016/0014-5793(91)80214-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the presence of specific binding sites for endothelin (ET) and the effect of ET on cytosolic free Ca2+ concentration ([Ca2+]i) in murine thioglycolate-activated peritoneal macrophages. Scatchard analysis for binding experiments using [I-125]ET-1 or [I-125]ET-3 revealed the existence of a single class of binding sites. The binding parameters (k(d) and B(max)) for [I-125]ET-1 were almost identical to those for [I-125]ET-3. In addition, unlabeled 3 ET isopeptides (ET-1, ET-2 and ET-3) inhibited the specific binding of both ET-1 and ET-3 with similar inhibitory potencies. All 3 ET isopeptides caused an increase in [Ca2+]i in the same dose-dependent manner (0.01-100 nM). These results demonstrate the existence of an ET receptor with the same affinity for all isoforms that mediates the ET-induced intracellular Ca2+ mobilization in murine peritoneal macrophages.

引用

页码：103 / 106

页数：4

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