ESTABLISHMENT OF MONOCLONAL-ANTIBODIES AGAINST HUMAN ACIDIC FIBROBLAST GROWTH-FACTOR

被引:7
作者
ICHIMORI, Y [1 ]
KINOSHITA, Y [1 ]
WATANABE, T [1 ]
SENO, M [1 ]
KONDO, K [1 ]
机构
[1] TAKEDA CHEM IND LTD,DIV RES & DEV,BIOTECHNOL RES LABS,OSAKA 532,JAPAN
关键词
D O I
10.1016/S0006-291X(05)81233-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Four kinds of hybridomas secreting monoclonal antibodies (MAbs) against human acidic fibroblast growth factor (haFGF) were established using recombinant haFGF as an immunogen. The recognition sites of four MAbs designated AF1-52, 81, 114 and 1C10 for the haFGF molecule were examined by binding studies with synthetic polypeptides and with amino-terminal truncated forms of haFGF. These experiments suggested that AF1-52, 114, and 1C10 MAbs recognize epitopes within the 1-5, 44-132 and 6-43 amino acid sequences, respectively. However, the epitope recognized by the AF1-81 MAb could not be determined. The sandwich EIA method constructed with these MAbs was sensitive to 1.5 pg/well of haFGF and had no cross-reactivity with human basic FGF, bovine aFGF or the hst-1 gene product. © 1991 Academic Press, Inc.
引用
收藏
页码:291 / 297
页数:7
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