学术探索
学术期刊
新闻热点
数据分析
智能评审

CHORIONIC VILLUS METAPHASE CHROMOSOMES AND INTERPHASE NUCLEI ANALYZED BY CHROMOSOMAL INSITU SUPPRESSION (CISS) HYBRIDIZATION

被引：13
作者：
KLEVER, M
GRONDGINSBACH, CJ
HAGER, HD
SCHROEDERKURTH, TM
机构：
[1] Institut Für Humangenetik Und Anthropologie, Heidelberg
来源：
PRENATAL DIAGNOSIS | 1992年 / 12卷 / 01期
关键词：
1ST-TRIMESTER PRENATAL CYTOGENETICAL DIAGNOSIS; CHROMOSOMAL INSITU SUPPRESSION (CISS) HYBRIDIZATION; INTERPHASE CYTOGENETICS;
D O I：
10.1002/pd.1970120108
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Metaphase chromosomes and interphase nuclei of chorionic villus samples (CVS) in five cases were studied after treatment with trypsin and post-fixation in formaldehyde by chromosomal in situ suppression (CISS) hybridization. Our modified protocol enables the use of in situ hybridization techniques on CVS preparations after 42 h of culture. A balanced translocation and trisomy 13 were identified with the aid of CISS hybridization.
引用
收藏
页码：53 / 59
页数：7
相关论文
共 18 条
[1]   A COLLABORATIVE STUDY OF THE SEGREGATION OF INHERITED CHROMOSOME STRUCTURAL REARRANGEMENTS IN 1356 PRENATAL DIAGNOSES [J].
BOUE, A ;
GALLANO, P .
PRENATAL DIAGNOSIS, 1984, 4 :45-67
[2]   RAPID INTERPHASE AND METAPHASE ASSESSMENT OF SPECIFIC CHROMOSOMAL CHANGES IN NEUROECTODERMAL TUMOR-CELLS BY INSITU HYBRIDIZATION WITH CHEMICALLY MODIFIED DNA PROBES [J].
CREMER, T ;
TESIN, D ;
HOPMAN, AHN ;
MANUELIDIS, L .
EXPERIMENTAL CELL RESEARCH, 1988, 176 (02) :199-220
[3]   DETECTION OF CHROMOSOME-ABERRATIONS IN METAPHASE AND INTERPHASE TUMOR-CELLS BY INSITU HYBRIDIZATION USING CHROMOSOME-SPECIFIC LIBRARY PROBES [J].
CREMER, T ;
LICHTER, P ;
BORDEN, J ;
WARD, DC ;
MANUELIDIS, L .
HUMAN GENETICS, 1988, 80 (03) :235-246
[4]  
EMMERICH P, 1989, LAB INVEST, V61, P235
[5]   DIRECT FETAL CHROMOSOME-STUDIES FROM CHORIONIC VILLI [J].
FLORI, E ;
NISAND, I ;
FLORI, J ;
DELLENBACH, P ;
RUCH, JV .
PRENATAL DIAGNOSIS, 1985, 5 (04) :287-289
[6]  
GROND CJ, 1983, EUR J CELL BIOL, V31, P150
[7]  
JAUCH A, 1990, HUM GENET, V85, P145
[8]  
KLEVER M, 1991, HUM GENET, V86, P484
[9]   DELINEATION OF INDIVIDUAL HUMAN-CHROMOSOMES IN METAPHASE AND INTERPHASE CELLS BY INSITU SUPPRESSION HYBRIDIZATION USING RECOMBINANT DNA LIBRARIES [J].
LICHTER, P ;
CREMER, T ;
BORDEN, J ;
MANUELIDIS, L ;
WARD, DC .
HUMAN GENETICS, 1988, 80 (03) :224-234
[10]  
LICHTER P, 1990, MOL GENETICS CHROMOS, P69
12