IMPROVING MULTIPLE ISOMORPHOUS REPLACEMENT PHASING BY HEAVY-ATOM REFINEMENT USING SOLVENT-FLATTENED PHASES

被引：27

作者：

ROULD, MA

PERONA, JJ

STEITZ, TA

机构：

[1] MIT,HOWARD HUGHES MED INST,DEPT MED,CAMBRIDGE,MA 02139

[2] YALE UNIV,DEPT MOLEC BIOPHYS & BIOCHEM,NEW HAVEN,CT 06511

[3] YALE UNIV,HOWARD HUGHES MED INST,NEW HAVEN,CT 06511

来源：

ACTA CRYSTALLOGRAPHICA SECTION A | 1992年 / 48卷

关键词：

D O I：

10.1107/S0108767392003404

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Solvent flattening of macromolecular MIR electron density maps is frequently used to improve the quality of the phases and the interpretability of resultant electron density maps. A new method is presented by which the heavy-atom parameters of isomorphous derivatives are refined against these same solvent-flattened phases and is shown to enhance convergence of the parameters by decoupling heavy-atom-parameter adjustment from parent-phase calculation. This approach is described here in the first example of its application in the solution of the glutaminyl-tRNA synthetase-tRNA(Gln)-ATP co-crystal structure.

引用

页码：751 / 756

页数：6

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