COMPUTATIONAL APPROACH TOWARDS THE 3-DIMENSIONAL STRUCTURE OF ESCHERICHIA-COLI TYROSINE AMINOTRANSFERASE

被引：14

作者：

JAGER, J ^{[1
]}

SOLMAJER, T ^{[1
]}

JANSONIUS, JN ^{[1
]}

机构：

[1] UNIV BASEL,BIOCTR,DEPT STRUCT BIOL,KLINGELBERGSTR 70,CH-4056 BASEL,SWITZERLAND

来源：

FEBS LETTERS | 1992年 / 306卷 / 2-3期

关键词：

TYROSINE AMINOTRANSFERASE; COMPUTER MODELING; X-RAY STRUCTURE; ASPARTATE AMINOTRANSFERASE; MUTANT ENZYME; ESCHERICHIA-COLI;

D O I：

10.1016/0014-5793(92)81007-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a new model for E. coli tyrosine aminotransferase based on the X-ray structures of the wild type and Val39Leu mutant of E. coli aspartate aminotransferase and computer simulation studies. Active site characteristics of the model are correlated with experimental observations on the specificity of these enzymes towards aromatic/dicarboxylic acid substrates.

引用

页码：234 / 238

页数：5

共 21 条

[1]

Braunstein AE., 1973, ENZYMES, V3d, P379, DOI [10.1016/S1874-6047%2808%2960122-5, DOI 10.1016/S1874-6047%2808%2960122-5]

[2]

BRUNGER AT, 1990, XPLOR MANUAL

[3]

Chou P Y, 1978, Adv Enzymol Relat Areas Mol Biol, V47, P45

[4]

Christen P., 1985, TRANSAMINASES

[5] THE CLONING AND SEQUENCE-ANALYSIS OF THE ASPC AND TYRB GENES FROM ESCHERICHIA-COLI-K12 - COMPARISON OF THE PRIMARY STRUCTURES OF THE ASPARTATE-AMINOTRANSFERASE AND AROMATIC AMINOTRANSFERASE OF ESCHERICHIA-COLI WITH THOSE OF THE PIG ASPARTATE-AMINOTRANSFERASE ISOENZYMESN [J].

FOTHERINGHAM, IG ;

DACEY, SA ;

TAYLOR, PP ;

SMITH, TJ ;

HUNTER, MG ;

FINLAY, ME ;

PRIMROSE, SB ;

PARKER, DM ;

EDWARDS, RM .

BIOCHEMICAL JOURNAL, 1986, 234 (03) :593-604

[6] ANALYSIS OF ACCURACY AND IMPLICATIONS OF SIMPLE METHODS FOR PREDICTING SECONDARY STRUCTURE OF GLOBULAR PROTEINS [J].

GARNIER, J ;

OSGUTHORPE, DJ ;

ROBSON, B .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 120 (01) :97-120

[7] REPLACEMENT OF AN INTERDOMAIN RESIDUE VAL39 OF ESCHERICHIA-COLI ASPARTATE-AMINOTRANSFERASE AFFECTS THE CATALYTIC COMPETENCE WITHOUT ALTERING THE SUBSTRATE-SPECIFICITY OF THE ENZYME [J].

HAYASHI, H ;

KURAMITSU, S ;

KAGAMIYAMA, H .

JOURNAL OF BIOCHEMISTRY, 1991, 109 (05) :699-704

[8] CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF AN ASPARTATE-AMINOTRANSFERASE MUTANT FROM ESCHERICHIA-COLI [J].

JAGER, J ;

KOHLER, E ;

TUCKER, P ;

SAUDER, U ;

HOUSLEYMARKOVIC, Z ;

FOTHERINGHAM, I ;

EDWARDS, M ;

HUNTER, M ;

KIRSCHNER, K ;

JANSONIUS, JN .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 209 (03) :499-501

[9]

JAGER J, 1991, THESIS U BASEL

[10]

JAGER J, 1990, 8TH P INT S VIT B6 C, P117

← 1 2 3 →