IMPROVED TECHNIQUE FOR IDENTIFICATION AND ENUMERATION OF METHANOGENIC BACTERIAL COLONIES ON ROLL TUBES BY EPIFLUORESCENCE MICROSCOPY

被引：8

作者：

KATAOKA, N

TOKIWA, Y

TAKEDA, K

机构：

[1] MINIST INT TRADE & IND,AGCY IND SCI & TECHNOL,FERMENTAT RES INST,DIV CHEM ECOL,1-1-3 HIGASHI,TSUKUBA,IBARAKI 305,JAPAN

[2] EBARA CORP,DEPT DEV & PLANNING,MINATO KU,TOKYO 108,JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 12期

关键词：

D O I：

10.1128/AEM.57.12.3671-3673.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Methanogenic fluorescent colonies can be clearly identified on roll tubes by using an epifluorescence microscope equipped with a x 2 objective. Methanogenic and nonmethanogenic colonies could be counted in roll tubes prepared from methanogenic enrichment cultures. Late-developing colonies appearing after 25 days of incubation were mainly methanogenic.

引用

页码：3671 / 3673

页数：3

共 13 条

[1]

AZUMA R, 1970, 1ST P INT C CULT COL, P493

[2] METHANOGENS - RE-EVALUATION OF A UNIQUE BIOLOGICAL GROUP [J].

BALCH, WE ;

FOX, GE ;

MAGRUM, LJ ;

WOESE, CR ;

WOLFE, RS .

MICROBIOLOGICAL REVIEWS, 1979, 43 (02) :260-296

[3] METHANOGENESIS FROM ACETATE - ENRICHMENT STUDIES [J].

BARESI, L ;

MAH, RA ;

WARD, DM ;

KAPLAN, IR .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1978, 36 (01) :186-197

[4] ISOLATION AND PROPERTIES OF A FLUORESCENT COMPOUND, FACTOR 420, FROM METHANOBACTERIUM STRAIN MOH [J].

CHEESEMAN, P ;

TOMSWOOD, A ;

WOLFE, RS .

JOURNAL OF BACTERIOLOGY, 1972, 112 (01) :527-+

[5] IMPROVED IDENTIFICATION OF METHANOGENIC BACTERIA BY FLUORESCENCE MICROSCOPY [J].

DODDEMA, HJ ;

VOGELS, GD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1978, 36 (05) :752-754

[6] NEW METHOD FOR ISOLATION AND IDENTIFICATION OF METHANOGENIC BACTERIA [J].

EDWARDS, T ;

MCBRIDE, BC .

APPLIED MICROBIOLOGY, 1975, 29 (04) :540-545

[7] ANALYSIS OF PLATE-COUNT DATA OF BACTERIA IN NATURAL ENVIRONMENTS [J].

HATTORI, T .

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 1982, 28 (01) :13-22

[8]

HATTORI T, 1988, VIABLE COUNT QUANTIT, P43

[9]

Hungate R. E., 1969, METHODS MICROBIOLOGY, V3, P117, DOI [10.1016/S0580-9517(08)70503-8, DOI 10.1016/S0580-9517(08)70503-8]

[10] ANALYSIS OF COLONY-FORMING CURVES OF SOIL BACTERIA [J].

ISHIKURI, S ;

HATTORI, T .

SOIL SCIENCE AND PLANT NUTRITION, 1987, 33 (03) :355-362

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