PLASMIC MEMBRANES OF HEPATOCYTES AND ADRENOCORTICOCYTES IN RATS OF DIFFERENT AGES - EFFECT OF TESTOSTERONE

被引:7
作者
FROLKIS, VV
KOBZAR, AL
TJUKHTIN, GM
机构
[1] Institute of Gerontology, Kiev
关键词
AGING; HEPATOCYTE; ADRENOCORTICOCYTE; TESTOSTERONE; HYPERPOLARIZATION; NA; K-ATPASE; HYPERPOLARIZATION FACTOR;
D O I
10.1016/0047-6374(92)90135-Z
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Experiments on adult (6-8-month-old and 26-28-month-old) Wistar rats revealed the hyperpolarization of plasmic membranes and activation of Na,K-ATPase of adrenocorticocytes in animals of both age groups and of hepatocytes of adult rats. No effect of testosterone was observed on the level of membrane potential and the activity of Na,K-ATPase of hepatocytes of old rats. The effect of testosterone was prevented by inhibitors of protein biosynthesis (actinomycin D and cycloheximide) and a specific inhibitor of Na,K-ATPase (ouabain), but not by K+-channel blocker 2-aminopyridine. Testosterone was assumed to synthesize the specific factor, capable of activating Na,K-ATPase of plasmic membranes. The cytosole of hepatocytes and the blood serum of adult testosterone-treated rats activated the Na,K-ATPase of isolated plasmic membranes of hepatocytes of adult and old intact rats. During aging there was a decrease in the capacity of cells to synthesize the specific factor, which activated Na,K-ATPase of plasmic membranes.
引用
收藏
页码:187 / 194
页数:8
相关论文
共 12 条
[1]  
FROLKIS VV, 1981, AGEING NEUROHUMORAL
[2]  
FROLKIS VV, 1980, PHYSL J, V26, P558
[3]   TESTOSTERONE UPTAKE BY PROSTATIC TISSUE FROM YOUNG AND OLD RATS [J].
GHANADIAN, R ;
FOTHERBY, K .
GERONTOLOGIA, 1975, 21 (04) :211-215
[4]  
GORBAN EN, 1979, PHYSL J, V24, P395
[5]   REPRODUCTIVE HORMONES IN AGING MEN .1. MEASUREMENT OF SEX STEROIDS, BASAL LUTEINIZING-HORMONE, AND LEYDIG-CELL RESPONSE TO HUMAN CHORIONIC-GONADOTROPIN [J].
HARMAN, SM ;
TSITOURAS, PD .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1980, 51 (01) :35-40
[6]  
KOSTUK PG, 1960, MICROELECTRODE TECNI
[7]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[8]  
MATHEWS EK, 1967, J PHYSL, V189, P149
[9]   SITE OF DIPHOSPHOINOSITIDE SYNTHESIS IN RAT LIVER [J].
MICHELL, RH ;
HAWTHORN.JN .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1965, 21 (04) :333-&
[10]   AGE-RELATED DECLINE OF PLASMA BIOAVAILABLE TESTOSTERONE IN ADULT MEN [J].
NAHOUL, K ;
ROGER, M .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1990, 35 (02) :293-299