3-DIMENSIONAL STRUCTURE OF A MEMBRANE-CONTAINING VIRUS

被引:167
作者
PAREDES, AM
BROWN, DT
ROTHNAGEL, R
CHIU, W
SCHOEPP, RJ
JOHNSTON, RE
PRASAD, BVV
机构
[1] UNIV TEXAS,CELL RES INST,AUSTIN,TX 78713
[2] BAYLOR COLL MED,WM KECK CTR COMPUTAT BIOL,HOUSTON,TX 77030
[3] UNIV N CAROLINA,SCH MED,DEPT MICROBIOL & IMMUNOL,CHAPEL HILL,NC 27599
[4] UNIV TEXAS,DEPT MICROBIOL,AUSTIN,TX 78713
[5] BAYLOR COLL MED,VERNA & MARRS MCLEAN DEPT BIOCHEM,HOUSTON,TX 77030
关键词
D O I
10.1073/pnas.90.19.9095
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The structure of Sindbis virus was determined by electron cryomicroscopy. The virion contains two icosahedral shells of viral-encoded proteins separated by a membrane bilayer of cellular origin. The three-dimensional structure of the ice-embedded intact Sindbis virus, reconstructed from electron images, unambiguously shows that proteins in both shells are arranged with the same icosahedral lattice of triangulation number T = 4. These studies also provide structural evidence of contact between the glycoprotein and the nucleocapsid protein across the membrane bilayer. The structural organization of Sindbis virus has profound implications for the morphogenesis of the alphaviruses. The observed interactions confirm stoichiometric and specific protein associations that may be crucial for virion stability and predict a mechanism for assembly.
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页码:9095 / 9099
页数:5
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