AFFINITY PRECIPITATION OF TRYPSIN WITH SOYBEAN TRYPSIN-INHIBITOR LINKED EUDRAGIT S-100

被引：16

作者：

KUMAR, A ^{[1
]}

GUPTA, MN ^{[1
]}

机构：

[1] INDIAN INST TECHNOL, HAUZ KHAS, DEPT CHEM, NEW DELHI 110016, INDIA

来源：

JOURNAL OF BIOTECHNOLOGY | 1994年 / 37卷 / 02期

关键词：

AFFINITY PRECIPITATION; BENZAMIDINE; EUDRAGIT; 8-100; SOYBEAN TRYPSIN INHIBITOR; TRYPSIN;

D O I：

10.1016/0168-1656(94)90010-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Soybean trypsin inhibitor linked to Eudragit S-100 was used for the affinity precipitation of trypsin. Polymer and ligand concentrations used in conjugate preparation showed remarkable effect on the trypsin recovery. Trypsin precipitation efficiency amounted to 89% and recovery was 74%. The final purification of relatively crude commercial trypsin resulted in 1.85-fold purification. The SDS-PAGE analysis indicated significant purification. The precipitated enzyme activity was around 96% and recovered enzyme activity was 83%.

引用

页码：185 / 189

页数：5

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