Localization of peroxisomal 3-oxoacyl-CoA thiolase in particles of varied density in rat liver: Implications for peroxisome biogenesis

被引:23
作者
vanRoermund, CWT
vandenBerg, M
Wanders, RJA
机构
[1] UNIV HOSP AMSTERDAM,DEPT CLIN CHEM,1105 AZ AMSTERDAM,NETHERLANDS
[2] UNIV HOSP AMSTERDAM,DEPT PEDIAT,1105 AZ AMSTERDAM,NETHERLANDS
[3] UNIV AMSTERDAM,ACAD MED CTR,E C SLATER INST,DEPT BIOCHEM,1105 AZ AMSTERDAM,NETHERLANDS
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1995年 / 1245卷 / 03期
关键词
microbody; fatty acid; biogenesis; peroxisome;
D O I
10.1016/0304-4165(95)00099-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this paper we report on the subcellular localization of peroxisomal thiolase in rat liver using density-gradient centrifugation and immunoelectron microscopy. The results obtained show that peroxisomes display great biochemical heterogeneity and can not be regarded as one homogeneous population of particles. We conclude that rat liver contains at least three distinct populations of peroxisomes, which are present both in normal-fed rats as well in rats treated with a plasticizer, di-(2-ethylexyl)phthalate, known to induce peroxisomes. The following types of peroxisomes could be discerned: (1) Low-density peroxisomal particles containing 69-kDa peroxisomal membrane protein (PMP), dihydroxyacetonephosphate acyltransferase (DHAPAT) and the precursor form of peroxisomal thiolase (44-kDa). (2) Intermediate-density peroxisomal particles containing 69-kDa peroxisomal membrane protein, dihydroxyacetonephosphate acyltransferase, both 41-kDa (mature) and 44-kDa (immature) peroxisomal thiolase, catalase and D-aminoacid oxidase. (3) High-density peroxisomes containing 69-kDa peroxisomal membrane protein, dihydroxyacetonephosphate acyltransferase, 41-kDa thiolase, catalase and D-aminoacid oxidase.
引用
收藏
页码:348 / 358
页数:11
相关论文
共 52 条
[1]   LOW-DENSITY PARTICLES (W-PARTICLES) CONTAINING CATALASE IN ZELLWEGER SYNDROME AND NORMAL FIBROBLASTS [J].
AIKAWA, J ;
CHEN, WW ;
KELLEY, RI ;
TADA, K ;
MOSER, HW ;
CHEN, GL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) :10084-10088
[2]   PARTIAL DISASSEMBLY OF PEROXISOMES [J].
ALEXSON, SEH ;
FUJIKI, Y ;
SHIO, H ;
LAZAROW, PB .
JOURNAL OF CELL BIOLOGY, 1985, 101 (01) :294-304
[3]   DEHYDROGENASES OF THE PENTOSE-PHOSPHATE PATHWAY IN RAT-LIVER PEROXISOMES [J].
ANTONENKOV, VD .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 183 (01) :75-82
[4]   TISSUE FRACTIONATION STUDIES .5. ASSOCIATION OF ACID PHOSPHATASE WITH A SPECIAL CLASS OF CYTOPLASMIC GRANULES IN RAT LIVER [J].
APPELMANS, F ;
WATTIAUX, R ;
DUVE, CD .
BIOCHEMICAL JOURNAL, 1955, 59 (03) :438-445
[5]   ABERRANT SUBCELLULAR-LOCALIZATION OF PEROXISOMAL 3-KETOACYL-COA THIOLASE IN THE ZELLWEGER SYNDROME AND RHIZOMELIC CHONDRODYSPLASIA PUNCTATA [J].
BALFE, A ;
HOEFLER, G ;
CHEN, WW ;
WATKINS, PA .
PEDIATRIC RESEARCH, 1990, 27 (03) :304-310
[6]  
BEIER K, 1988, EUR J CELL BIOL, V46, P383
[7]   PEROXISOMAL BETA-OXIDATION ENZYME PROTEINS IN ADRENOLEUKODYSTROPHY - DISTINCTION BETWEEN X-LINKED ADRENOLEUKODYSTROPHY AND NEONATAL ADRENOLEUKODYSTROPHY [J].
CHEN, WW ;
WATKINS, PA ;
OSUMI, T ;
HASHIMOTO, T ;
MOSER, HW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (05) :1425-1428
[8]   PEROXISOMES (MICROBODIES AND RELATED PARTICLES) [J].
DEDUVE, C ;
BAUDHUIN, P .
PHYSIOLOGICAL REVIEWS, 1966, 46 (02) :323-+
[9]   IMMUNOCYTOCHEMICAL DETECTION OF PEROXISOMAL BETA-OXIDATION ENZYMES IN CRYOSTAT AND PARAFFIN SECTIONS OF HUMAN POST-MORTEM LIVER [J].
ESPEEL, M ;
HASHIMOTO, T ;
DECRAEMER, D ;
ROELS, F .
HISTOCHEMICAL JOURNAL, 1990, 22 (01) :57-62
[10]  
FLATMARK T, 1981, EUR J CELL BIOL, V24, P62