METABOLIC-FATE OF AROMATIC-HYDROCARBONS IN AQUATIC ORGANISMS - ANALYSIS OF METABOLITES BY THIN-LAYER CHROMATOGRAPHY AND HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

Aquatic organisms convert aromatic hydrocarbons into a variety of conjugated and nonconjugated derivatives. Analytical techniques based on thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC) were employed to separate, identify and quantitate individual metabolites from fish exposed to radiolabeled naphthalene and 2, 6- dimethylnaphthalene. Significant differences in profiles of individual metabolites were found in relation to the type of biological sample analyzed. Liver of naphthalene-exposed salmonids contained eight polar derivatives, as shown by HPLC. Two nonconjugates (1-naphthol and l, 2-dihydro-l, 2-dihydroxynaphthalene) and three conjugates (1-naphthyl glucuronic acid, 1-naphthyl sulfate and 1-naphthyl glucoside) were identified. HPLC revealed that brain of 3H- naphthalene-exposed trout contained essentially the nonconjugated derivatives, 1-naphthol and l, 2-dihydro-l, 2-dihydroxynaphthalene. TLC showed that the metabolities from trout urine were 1-naphthol, l, 2-dihydro-l, 2-dihydroxynaphthalene, and 1-naphthyl glucuronic acid (99% of the total metabolites detected). Major components of the metabolite fractions of tissues and biological fluids were 1, 2-dihydro-l, 2-dihydroxy and glucuronic acid derivatives. Dihydrodiol derivatives arise from the corresponding arene oxides, some of which have been shown to be cytotoxic to certain mammalian systems. © 1979, Taylor & Francis Group, LLC. All rights reserved.