REVERSIBLE THERMAL UNFOLDING OF BACILLUS-SUBTILIS LEVANSUCRASE IS MODULATED BY FE-3+ AND CA-2+

被引：22

作者：

CHAMBERT, R

PETITGLATRON, MF

机构：

[1] Institut Jacques Monod, CNRS, Université Paris VII, 75251 Paris Cedex, Tour 43-2, place Jussieu

来源：

FEBS LETTERS | 1990年 / 275卷 / 1-2期

关键词：

Bacillus subtilis; Levansucrase; Protein secretion; Thermal unfolding;

D O I：

10.1016/0014-5793(90)81439-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The equilibrium transition curves for thermal unfolding of levansucrase were established at several pH values. At pH 7 and within the temperature range of bacterial growth, the unfolded from is predominant. However, under such conditions, refolding is promoted by the only addition of Ca2+ or Fe3+. We propose that the tertiary structure flexibility of levansucrase plays a key role in its secretion process. © 1990.

引用

页码：61 / 64

页数：4

共 12 条

[1] THE MOLTEN GLOBULE STATE IS INVOLVED IN THE TRANSLOCATION OF PROTEINS ACROSS MEMBRANES [J].