HIGHLY SENSITIVE ENHANCED CHEMILUMINESCENCE IMMUNODETECTION METHOD FOR HERPES-SIMPLEX VIRUS TYPE-2 WESTERN IMMUNOBLOT

被引：40

作者：

DALESSIO, J ^{[1
]}

ASHLEY, R ^{[1
]}

机构：

[1] UNIV WASHINGTON,DEPT LAB MED,DIV VIROL,ZC-10,SEATTLE,WA 98195

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 04期

关键词：

D O I：

10.1128/JCM.30.4.1005-1007.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Three Western blot (immunoblot) methods for detecting antibodies to herpes simplex virus type 2 were compared: (i) nitrocellulose blots with 4-chloro-1-naphthol immunostaining (4CN-WB); (ii) polyvinylidene difluoride (PVDF) blots with 3,3',5,5'-tetramethylbenzidene immunostaining (TMB-WB); and (iii) PVDF blots with enhanced chemiluminescence (ECL-WB). TMB-WB was 10-fold more sensitive than 4CN-WB, while ECL-WB was as much as 500-fold more sensitive.

引用

页码：1005 / 1007

页数：3

共 18 条

[1] INABILITY OF ENZYME IMMUNOASSAYS TO DISCRIMINATE BETWEEN INFECTIONS WITH HERPES-SIMPLEX VIRUS TYPE-1 AND TYPE-2 [J].

ASHLEY, R ;

CENT, A ;

MAGGS, V ;

NAHMIAS, A ;

COREY, L .

ANNALS OF INTERNAL MEDICINE, 1991, 115 (07) :520-526

[2] DIFFERENTIAL EFFECT OF SYSTEMIC ACYCLOVIR TREATMENT OF GENITAL HSV-2 INFECTIONS ON ANTIBODY-RESPONSES TO INDIVIDUAL HSV-2 PROTEINS [J].

ASHLEY, R ;

MACK, K ;

CRITCHLOW, C ;

SHURTLEFF, M ;

COREY, L .

JOURNAL OF MEDICAL VIROLOGY, 1988, 24 (03) :309-320

[3] GENITAL HERPES INFECTIONS [J].

ASHLEY, RL .

CLINICS IN LABORATORY MEDICINE, 1989, 9 (03) :405-420

[4] COMPARISON OF WESTERN BLOT (IMMUNOBLOT) AND GLYCOPROTEIN-G-SPECIFIC IMMUNODOT ENZYME ASSAY FOR DETECTING ANTIBODIES TO HERPES-SIMPLEX VIRUS TYPE-1 AND TYPE-2 IN HUMAN-SERA [J].

ASHLEY, RL ;

MILITONI, J ;

LEE, F ;

NAHMIAS, A ;

COREY, L .

JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (04) :662-667

[5]

ASHLEY RL, 1984, J CLIN INVEST, V73, P681, DOI 10.1172/JCI111260

[6] USE OF DENSITOMETRIC ANALYSIS FOR INTERPRETING HSV SEROLOGIES BASED ON WESTERN-BLOT [J].

ASHLEY, RL ;

MILITONI, J .

JOURNAL OF VIROLOGICAL METHODS, 1987, 18 (2-3) :159-168

[7]

Ashley RL, 1989, DIAGNOSTIC PROCEDURE, V6th, P265

[8] ANTIBODY-RESPONSE TO TYPE-COMMON AND TYPE-UNIQUE EPITOPES OF HERPES-SIMPLEX VIRUS POLYPEPTIDES [J].

BERNSTEIN, DI ;

BRYSON, YJ ;

LOVETT, MA .

JOURNAL OF MEDICAL VIROLOGY, 1985, 15 (03) :251-263

[9] DETECTION OF ASYMPTOMATIC INITIAL HERPES-SIMPLEX VIRUS (HSV) INFECTIONS IN ANIMALS IMMUNIZED WITH SUBUNIT HSV GLYCOPROTEIN VACCINES [J].

BERNSTEIN, DI ;

ASHLEY, RL ;

STANBERRY, LR ;

MYERS, MG .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (01) :11-15

[10] THE EFFECTS OF ACYCLOVIR ON ANTIBODY-RESPONSE TO HERPES-SIMPLEX VIRUS IN PRIMARY GENITAL HERPETIC INFECTIONS [J].

BERNSTEIN, DI ;

LOVETT, MA ;

BRYSON, YJ .

JOURNAL OF INFECTIOUS DISEASES, 1984, 150 (01) :7-13

← 1 2 →