LANGMUIR-BLODGETT-FILM BASED MEMBRANE FOR DNA-PROBE BIOSENSOR

被引：13

作者：

KARYMOV, MA ^{[1
]}

KRUCHININ, AA ^{[1
]}

TARANTOV, YA ^{[1
]}

BALOVA, IA ^{[1
]}

REMISOVA, LA ^{[1
]}

SUKHODOLOV, NG ^{[1
]}

YANKLOVICH, AI ^{[1
]}

YORKIN, AM ^{[1
]}

机构：

[1] ST PETERSBURG UNIV,DEPT GENET,ST PETERSBURG 198904,USSR

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1992年 / 6卷 / 1-3期

关键词：

D O I：

10.1016/0925-4005(92)80057-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Covalent attachment of DNA to diacetylene film produced on the surface of oxidized silicon by the Langmuir-Blodgett technique has been studied. Coupling of phage M13 DNA with polymer film was carried out by the carbodiimide method and by the formation of linker structures. Sorption capacity and hybridization efficiency determined using the radioactive label P-32 were about 10-20 pg mm-2 and near 100%, respectively.

引用

页码：208 / 210

页数：3