MOLECULAR-CLONING AND DEVELOPMENTAL EXPRESSION OF A HUMAN KIDNEY S-ADENOSYLMETHIONINE SYNTHETASE

被引：75

作者：

HORIKAWA, S

TSUKADA, K

机构：

[1] Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo, 101, Kanda-surugadai

来源：

FEBS LETTERS | 1992年 / 312卷 / 01期

关键词：

S-ADENOSYLMETHIONINE; ISOZYME; CDNA SEQUENCE; HUMAN KIDNEY;

D O I：

10.1016/0014-5793(92)81405-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA clones encoding the human kidney S-adenosylmethionine synthetase (kidney-type isozyme) were isolated. The amino acid sequence deduced from the cDNA indicates that this enzyme contains 395 amino acids and has a molecular mass of 43,660 Da. The predicted amino acid sequence of this protein shares 84% similarity with that of human liver S-adenosylmethionine synthetase (liver-type isozyme). In addition, the developmental expression of these two isozyme mRNAs has been studied in the human liver using the reverse transcription-polymerase chain reaction (RT-PCR).

引用

页码：37 / 41

页数：5

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