ENUMERATION OF VIBRIO-VULNIFICUS ON MEMBRANE FILTERS WITH A FLUORESCENTLY LABELED OLIGONUCLEOTIDE PROBE SPECIFIC FOR KINGDOM-LEVEL 16S RIBOSOMAL-RNA SEQUENCES

被引：16

作者：

HEIDELBERG, JF

ONEILL, KR

JACOBS, D

COLWELL, RR

机构：

[1] UNIV MARYLAND,MARINE ESTUARINE ENVIRONM SCI PROGRAM,COLL PK,MD 20742

[2] UNIV MARYLAND,MARYLAND SEA GRANT COLL,COLL PK,MD 20742

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 10期

关键词：

D O I：

10.1128/AEM.59.10.3474-3476.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Vibrio vulnificus was enumerated on membrane filters after hybridization with a fluorescent oligonucleotide eubacterial probe. Cells were hybridized in liquid buffer or directly on membrane filters. There was no significant difference between fluorescent oligonucleotide direct counts and acridine orange direct counts (P > 0.05). Liquid buffer hybridization was preferable to direct filter hybridization.

引用

收藏

页码：3474 / 3476

页数：3

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