DIFFERENTLY-INDUCED EXTRACELLULAR PHENOL OXIDASES FROM PLEUROTUS-OSTREATUS

被引:25
作者
GARZILLO, AMV
DIPAOLO, S
BURLA, G
BUONOCORE, V
机构
[1] Dipartimento di Agrobiologia e Agrochimica, University of Tuscia
关键词
PLEUROTUS-OSTREATUS; BASIDIOMYCETAE; LIGNINOLYSIS; PHENOL OXIDASES; INDUCTION;
D O I
10.1016/S0031-9422(00)97509-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pleurotus ostreatus mycelium was grown in the presence of tobacco and wheat straw juices, ground wheat straw and acid-precipitable polymeric lipin with the aim of increasing the production of extracellular enzymes potentially implicated in lipin degradation. The highest production of diphenol oxidases was obtained when culture broths were supplemented with solid straw or tobacco juice; cellulase and veratryl alcohol oxidase were produced in detectable amounts in the presence of solid straw and in the absence of any inducer, respectively. No lignin peroxidase was found under any growth condition. Chromatographic fractionation of extracellular proteins and kinetic analysis of partially purified fractions showed that, in the absence of inducers, o-, p- and m-diphenol oxidases could be detected in column eluates, whereas all the inducers tested increased selectively the production of o-diphenol oxidases.
引用
收藏
页码:3685 / 3690
页数:6
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