INTERACTIONS OF POLYAMINES IN THE MEASUREMENT OF FREE MAGNESIUM CONCENTRATION BY MAG-FURA-2 AND P-31-NMR

被引：11

作者：

GUNTHER, T

VORMANN, J

KONSTANCZAK, P

SCHAFER, A

机构：

[1] TECH UNIV BERLIN,IWAN N STRANSKI INST PHYS & THEORET CHEM,W-1000 BERLIN,GERMANY

[2] FREE UNIV BERLIN,INST ORGAN CHEM,W-1000 BERLIN,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1192卷 / 02期

关键词：

INTRACELLULAR FREE MAGNESIUM; MAGNESIUM ION; INTRACELLULAR; POLYAMINE; MAG-FURA-2; NMR; P-31-;

D O I：

10.1016/0005-2736(94)90129-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyamines, particularly spermine, in physiological concentrations interact with mag-fura-2 and the mag-fura-2/Mg2+ complex, resulting in reduced values of free Mg2+ concentration. Similarly, polyamines interact with ATP and MgATP. Thus, free Mg2+ concentration, as measured by P-31-NMR or mag-fura-2, is underestimated in the presence of polyamines, particularly of spermine.

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收藏

页码：281 / 285

页数：5

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