CLONING AND EXPRESSION OF EEL NATRIURETIC-PEPTIDE RECEPTOR-B AND COMPARISON WITH ITS MAMMALIAN COUNTERPARTS

被引:66
作者
KATAFUCHI, T
TAKASHIMA, A
KASHIWAGI, M
HAGIWARA, H
TAKEI, Y
HIROSE, S
机构
[1] TOKYO INST TECHNOL,DEPT BIOL SCI,YOKOHAMA 227,JAPAN
[2] UNIV TOKYO,OCEAN RES INST,PHYSIOL LAB,TOKYO 164,JAPAN
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 222卷 / 03期
关键词
D O I
10.1111/j.1432-1033.1994.tb18930.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A comparative study of the natriuretic-peptide receptor NPR-B was performed by cloning and expressing, in COS-1 cells, the NPR-B receptor subtype from the eel gill which exhibited a strong C-type-natriuretic-peptide(CNP)-induced guanylate cyclase activity. Like other mammalian NPR-B receptors, the eel NPR-B receptor consisted of a ligand-binding extracellular domain, a hydrophobic transmembrane domain, a kinase-like domain and a guanylate cyclase domain. Sequence comparison among the eel and mammalian receptors revealed a relatively low similarity (approximate to 44%) in the extracellular domain compared to a very high similarity (approximate to 84%) in the cytoplasmic regulatory and catalytic domains. This low similarity allowed identification of the amino acid residues or candidate regions important for the ligand-binding activity. RNase protection analysis of the eel NPR-B mRNA demonstrated that the message was predominantly expressed in the liver and atrium as well as in the gill with moderate-to-small amounts in the brain, ventricle, esophageal sphincter, stomach, posterior intestine and kidney. The high NPR-B mRNA levels in the liver, atrium and gill were found to decrease markedly when eels were transferred from fresh water to seawater and kept there for 2 weeks. Since similar changes are known to occur in the ligand CNP levels when eels are facing osmotic challenges, the CNP/NPR-B system appears to play an important role in their successful adaptation to salinity changes.
引用
收藏
页码:835 / 842
页数:8
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