Mesoderm Induction in Xenopus laevis Distinguishes Between the Various TGF-beta Isoforms

被引：61

作者：

Roberts, Anita B. ^{[1
]}

Kondaiah, Paturu ^{[1
]}

Rosa, Frederic ^{[2
]}

Watanabe, Shinichi ^{[1
]}

Good, Peter ^{[2
]}

Danielpour, David ^{[1
]}

Roche, Nanette S. ^{[1
]}

Rebbert, Martha L. ^{[2
]}

Dawid, Igor B. ^{[2
]}

Sporn, Michael B. ^{[1
]}

机构：

[1] NCI, Chemoprevent Lab, Bethesda, MD 20892 USA

[2] NICHHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA

来源：

GROWTH FACTORS | 1990年 / 3卷 / 04期

关键词：

TGF-beta; 3; Xenopus laevrs; mesoderm induction; isoform switching; transforming growth factor-beta;

D O I：

10.3109/08977199009003670

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Induction of mesoderm in ectodermal explants of Xeizopus laevis blastula embryos had previously been shown to respond selectively to TGF-beta s, with TGF-beta s 1 and 5 having no activity in this assay. As TGF-Bs 1, 2, and 3 are frequently coexpressed in tissues, we wished to examine the activity of TGF-beta 3 relative to that of TGF-beta s 1 and 2 in this assay as well as in other in uitro assays. We report here that when the activity of recombinant 'TGF-beta 3 is normalized to that of TGF-beta 1 in the assay for growth inhibition in CCL-64 cells, it is also equal to that of TGF-beta 1 in assays for stimulation of both anchorage-independent growth of rat NRK cells and chemotaxis of human monocytes. In contrast, in the assay for mesoderm induction, recombinant TGF-beta 3 is 10-fold more active than TGF-beta 2, inducing expression of muscle specific alpha-actin at concentrations as low as 1 ng/ml. These results suggest that more complex systems, in contrast to individual cell types, may respond selectively to the various TGF-beta isoforms and that there might be biological consequences of TGF-beta isoform switching in vivo.

引用

页码：277 / 286

页数：10

共 54 条

[1] LOCALIZED PRODUCTION OF TGF-BETA MESSENGER-RNA IN TUMOR PROMOTER-STIMULATED MOUSE EPIDERMIS [J].