CHIRAL CHLOROFORMATES AS TRANSPARENT REAGENTS FOR THE RESOLUTION OF METOPROLOL ENANTIOMERS BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY

被引：17

作者：

AHNOFF, M

CHEN, S

GREEN, A

GRUNDEVIK, I

机构：

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 506卷

关键词：

D O I：

10.1016/S0021-9673(01)91607-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ten chiral chloroformates were prepared and tested as derivatizing reagents, using metoprolol and tyrosine enantiomers as model compounds. Chloroformates derived from isosorbide and isomannide monoesters gave separation factors of 1.07-2.16 for tyrosine and 1.06-1.08 for metoprolol in reversed-phase liquid chromatography. With tert.-butyl 3-(chloroformoxy)butyrate as derivatizing reagent a separation factor of 1.10 and a resolution of 2.0 were obtained in 12 min for (R)_- and (S)-metoprolol. The reagent and its by-products did not interfere with the UV or fluorescence detection of metoprolol. © 1990.

引用

页码：593 / 599

页数：7

共 8 条

[1]

AHNOFF M, 1989, CHIRAL LIQUID CHROMA, P39

[2]

ARNOULD MC, 1980, ANALUSIS, V8, P76

[3] DETERMINATION OF METOPROLOL AND 2 MAJOR METABOLITES IN PLASMA AND URINE BY COLUMN LIQUID-CHROMATOGRAPHY AND FLUOROMETRIC DETECTION [J].