LOCALIZATION OF ZONA ANTIGEN IN BOVINE OVARY SECTIONS BY FLUORESCENT-ANTIBODY

Treatment of bovine ovary sections with rabbit antibovine zona serum followed by fluorescein‐conjugated goat antirabbit IgG produced a specific fluorescent staining only of the zonae pellucidae. Fluorescence was greater near the inner and outer surface of the zona, suggesting that either the same antigen occurs in higher concentration in these regions or that there is more than one antigen, the most immunogenic being located peripherally. In some atretic follicles fluorescent material appeared to diffuse into the degenerating oocyte and into the intercellular spaces of the cumulus oophorus. Copyright © 1979 Alan R. Liss, Inc.