DETECTION OF EPSTEIN-BARR-VIRUS DNA BY POLYMERASE CHAIN-REACTION IN LYMPH-NODE BIOPSIES FROM PATIENTS WITH ANGIOIMMUNOBLASTIC LYMPHADENOPATHY

被引：82

作者：

KNECHT, H

SAHLI, R

SHAW, P

MEYER, C

BACHMANN, E

ODERMATT, BF

BACHMANN, F

机构：

[1] CHU VAUDOIS, INST MICROBIOL, CH-1011 LAUSANNE, SWITZERLAND

[2] CHU VAUDOIS, INST PATHOL, CH-1011 LAUSANNE, SWITZERLAND

[3] ISREC, EPALINGES, SWITZERLAND

[4] UNIV HOSP ZURICH, INST PATHOL, CH-8091 ZURICH, SWITZERLAND

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1990年 / 75卷 / 04期

关键词：

D O I：

10.1111/j.1365-2141.1990.tb07807.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Summary. Epstein‐Barr virus DNA was detected by the polymerase chain reaction (PCR) in five lymph node biopsies from eight patients with diagnosis of angioimmunoblastic lymphadenopathy (AILD). Three pairs of specific primers detected EBV DNA sequences near the 5’end (Bam W region), the middle (BMRF 1 region) and the 3’end (Eco RI D region) of the viral genome with equal accuracy when 1 μg of DNA and 30 amplification cycles were used. When only 100 ng of DNA were screened with the BMRF 1 set of primers, a specific amplification product was still identifiable. The 593 base pair amplification product obtained using the Eco RI D set of primers was shown to contain an expected Sma I site at position 215, confirming the viral origin of sequence. Our findings indicate that lymph nodes of AILD patients frequently harbour the entire EBV genome at a high percentage of at least 1 viral copy per 15 000 human cells. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：610 / 614

页数：5

共 23 条

[1] ATTEMPTS TO DEMONSTRATE VIRUS-SPECIFIC SEQUENCES IN HUMAN TUMORS .4. EB VIRAL DNA IN EUROPEAN BURKITT-LYMPHOMA AND IMMUNOBLASTIC LYMPHADENOPATHY WITH EXCESSIVE PLASMACYTOSIS [J].