SCREENING METHOD FOR LARGE NUMBERS OF DYE-ADSORBENTS FOR ENZYME-PURIFICATION

被引：17

作者：

HONDMANN, DHA ^{[1
]}

VISSER, J ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN,DEPT GENET,MOLEC GENET SECT,DREYENLAAN 2,6703 HA WAGENINGEN,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 510卷

关键词：

D O I：

10.1016/S0021-9673(01)93749-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is described by means of which 96 different dye-adsorbent can be tested simultaneously for their ability to bind enzymes and to test their biospecific elution. Small amounts of cell-free extract applied to dye-adsorbents which are packed in a 96-well transplate cartridge. After biospecific elution, the amount of the eluted enzyme is tested in a microtitre plate assay. The method is illustrated by the purification of glycerol dehydrogenase (E.C. 1.1.1.72), 6-phosphogluconate dehydrogenase (E.C. 1.1.1.44) and glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49) from the hyphal fungus Aspergillus nidulans. © 1990.

引用

页码：155 / 164

页数：10

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