THERMODYNAMICS OF CRO PROTEIN DNA INTERACTIONS

被引:120
作者
TAKEDA, Y
ROSS, PD
MUDD, CP
机构
[1] NIH, NATL CTR RES RESOURCES, BIOMED ENGN & INSTRUMENTAT PROGRAM, BETHESDA, MD 20892 USA
[2] NIDDKD, MOLEC BIOL LAB, BETHESDA, MD 20892 USA
关键词
ENTHALPY; ENTROPY; HEAT CAPACITY CHANGE; CALORIMETRY; DNA SEQUENCE RECOGNITION;
D O I
10.1073/pnas.89.17.8180
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Using a highly sensitive pulsed-flow microcalorimeter, we have measured the changes in enthalpy and determined the thermodynamic parameters DELTA-H, DELTA-S-degrees, DELTA-G-degrees, and DELTA-C(p) for Cro protein-DNA association reactions. The reactions studied include sequence-nonspecific DNA association and sequence-specific DNA associations involving single- and multiple-base alterations and/or single-amino acid alteration mutants. (i) The association of Cro protein with nonspecific DNA at 15-degrees-C is characterized by DELTA-H = +4.4 kcal.mol-1 (1 cal = 4.18J), DELTA-S-degrees = 49 cal.mol-1.K-1, DELTA-G-degrees = -9.7 kcal.mol-1, and DELTA-C(p) congruent-to 0; the association this specific high-affinity operator OR3 DNA is characterized by DELTA-H = +0.8 kcal.mol-1, DELTA-S-degrees 59 cal.mol-1.K-1, DELTA-G-degrees = -16.1 kcal.mol-1, and DELTA-C(p) = -360 cal.mol-1.K-1, respectively. Both nonspecific and specific Cro-DNA associations are entropy-driven. (ii) Plots of DELTA-H vs. DELTA-C(p) and DELTA-S-degrees vs. DELTA-C(p) for the 20 association reactions studied fall into two correlation groups with linear slopes of +9.4 K and -20.5 K and of -0.03 and -0.14, respectively. These regression lines have common intercepts, at the DELTA-H and DELTA-S-degrees values of nonspecific association (where DELTA-C(p) congruent-to 0). The results suggest that there are, at least, two distinct conformational subclasses in specific Cro-DNA complexes, stabilized by different combinations of enthalpic and entropic contributions. The DELTA-G-degrees and DELTA-C(p) values form an approximately single linear correlation group as a consequence of compensatory contributions from DELTA-H and DELTA-S-degrees to DELTA-G-degrees and to DELTA-C(p). Cro protein-DNA associations share some similar thermodynamic properties with protein folding, but their overall energetics are quite different. Although the nonspecific complex is stabilized predominantly by electrostatic forces, it appears that H bonds, van der Waals contacts, hydrophobic effects, and charge interactions all contribute to the stability (DELTA-G-degrees and DELTA-C(p)) of the specific complex. (iii) The variations in the values of the thermodynamic parameters are in general accord with our knowledge of the structure of the Cro-DNA complex.
引用
收藏
页码:8180 / 8184
页数:5
相关论文
共 37 条
[1]   RECOGNITION OF A DNA OPERATOR BY THE REPRESSOR OF PHAGE-434 - A VIEW AT HIGH-RESOLUTION [J].
AGGARWAL, AK ;
RODGERS, DW ;
DROTTAR, M ;
PTASHNE, M ;
HARRISON, SC .
SCIENCE, 1988, 242 (4880) :899-907
[2]   STRUCTURE OF THE CRO REPRESSOR FROM BACTERIOPHAGE-LAMBDA AND ITS INTERACTION WITH DNA [J].
ANDERSON, WF ;
OHLENDORF, DH ;
TAKEDA, Y ;
MATTHEWS, BW .
NATURE, 1981, 290 (5809) :754-758
[3]   LAMBDA-PHAGE CRO-REPRESSOR - NON-SPECIFIC DNA-BINDING [J].
BOSCHELLI, F .
JOURNAL OF MOLECULAR BIOLOGY, 1982, 162 (02) :267-282
[4]  
BRENNAN RG, 1989, J BIOL CHEM, V264, P1903
[5]   PROTEIN DNA CONFORMATIONAL-CHANGES IN THE CRYSTAL-STRUCTURE OF A LAMBDA-CRO-OPERATOR COMPLEX [J].
BRENNAN, RG ;
RODERICK, SL ;
TAKEDA, Y ;
MATTHEWS, BW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (20) :8165-8169
[6]   NONSPECIFIC INTERACTION OF LAC REPRESSOR WITH DNA - ASSOCIATION REACTION DRIVEN BY COUNTERION RELEASE [J].
DEHASETH, PL ;
LOHMAN, TM ;
RECORD, MT .
BIOCHEMISTRY, 1977, 16 (22) :4783-4790
[7]   Apparent molal heat capacities of ammo acids and other organic compounds [J].
Edsall, JT .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1935, 57 :1506-1507
[8]  
Eftink M., 1980, BIOL MICROCALORIM, P343
[9]   ROLE OF THE HYDROPHOBIC EFFECT IN STABILITY OF SITE-SPECIFIC PROTEIN-DNA COMPLEXES [J].
HA, JH ;
SPOLAR, RS ;
RECORD, MT .
JOURNAL OF MOLECULAR BIOLOGY, 1989, 209 (04) :801-816
[10]   DNA RECOGNITION BY PROTEINS WITH THE HELIX-TURN-HELIX MOTIF [J].
HARRISON, SC ;
AGGARWAL, AK .
ANNUAL REVIEW OF BIOCHEMISTRY, 1990, 59 :933-969