KINETIC-ANALYSIS OF CELLULAR NUCLEOTIDES CORRELATED TO GROWTH AND TPA PRODUCTION IN PERFUSION CULTURES OF HUMAN FIBROBLAST CELLS

被引：2

作者：

NAM, YN

YU, HK

JEON, BC

DECKWER, WD

LEE, HY

机构：

[1] KANGWEON NATL UNIV,DEPT FOOD ENGN,CHUNCHON 200701,SOUTH KOREA

[2] SAMLL PHARMACEUT CO,CENT RES INST,SEOUL,SOUTH KOREA

[3] GESELL BIOTECHNOL FORSCH MBH,DIV BIOCHEM ENGN,W-3300 BRAUNSCHWEIG,GERMANY

来源：

CYTOTECHNOLOGY | 1993年 / 11卷 / 02期

关键词：

INTRACELLULAR NUCLEOTIDES; OXYGEN UPTAKE RATE; PERFUSION CULTIVATION; TPA PRODUCTION; HUMAN FIBROBLAST CELL LINE;

D O I：

10.1007/BF00749003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A maximum cell density of 8 x 10(6) viable human fibroblast cells/ml was obtained on microcarriers at a perfusion rate of 0.6 mL/min - a rate which maintained a quasi-steady state. The maximum tPA production was approximately 1.2 mug/ml and obtained when the cell density was relatively constant during the later periods of cultivation. Specific UTP and tPA production rates had a correlation factor of 0.85, and cell growth to oxygen consumption had an 0.92 correlation factor. ATP generation was strongly correlated to glutamine consumption, with a lower correlation to oxygen utilization.

引用

页码：143 / 148

页数：6

共 17 条

[1]

ATKINSON DE, 1977, CELLULAR ENERGY META

[2]

CHRISTMAN JK, 1977, PROTEINASES MAMMALIA, P91

[3]

DRAPER N, 1981, APPLIED REGRESSION A, P423

[4]

FRESHNEY RI, 1983, CULTURE ANIMAL CELLS, P199

[5]

GOERGEN JL, 1991, PRODUCTION OF BIOLOGICALS FROM ANIMAL CELLS IN CULTURE, P634

[6]

GRIFFITHS JB, 1987, ADV BIOCHEM ENG, V34, P155

[7] REGULATION OF ATP POOLS, RIBOSOMAL-RNA AND DNA-SYNTHESIS IN 3T3 CELLS IN RESPONSE TO SERUM OR HYPOXANTHINE [J].

GRUMMT, F ;

PAUL, D ;

GRUMMT, I .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 76 (01) :7-12

[8]

LEE KY, 1988, J APPL MICROBIOL BIO, V16, P282

[9] TRANSIENT RESPONSES OF HYBRIDOMA METABOLISM TO CHANGES IN THE OXYGEN-SUPPLY RATE IN CONTINUOUS CULTURE [J].

MILLER, WM ;

WILKE, CR ;

BLANCH, HW .

BIOPROCESS ENGINEERING, 1988, 3 (03) :103-111

[10]

MURPHREE S, 1974, EXP CELL RES, V83, P189

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