ISOLATION AND IDENTIFICATION OF CECROPIN ANTIBACTERIAL PEPTIDES FROM THE EXTRACELLULAR-MATRIX OF THE INSECT INTEGUMENT

被引：17

作者：

LEE, WJ ^{[1
]}

BREY, PT ^{[1
]}

机构：

[1] INST PASTEUR, UNITE ECOL SYST VECTORIELS, F-75724 PARIS 15, FRANCE

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 217卷 / 02期

关键词：

D O I：

10.1006/abio.1994.1113

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the screening and isolation of antibacterial peptides from the extracellular insect cuticle using ultrafiltration and reverse-phase high-performance liquid chromatography is presented. Using this method we were able to conveniently isolate cuticular antibacterial peptides belonging to the cecropin family in 1 day. The procedure should facilitate the rapid screening of antibacterial peptides or other bioactive peptides from extracellular matrices. (C) 1994 Aeademic Press, Inc.

引用

页码：231 / 235

页数：5

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