TOPOGRAPHY OF NUCLEIC ACID HELICES IN SOLUTIONS .14. A PMR STUDY OF INTERACTIONS OF REPORTER MOLECULES WITH POLYNUCLEOTIDES

This paper reports the preliminary results of the proton magnetic resonance (pmr) spectra of the free and nucleic acid-bound reporter molecules 2 and 3. {A figure is presented} It is found that the DNA and RNA-reporter 2 complexes result in an upfield shift and considerable line broadening for the c- and a-methyl signals as compared with the free reporter molecule. The chemical shift of the b-methyl signal remains unchanged for the free and the bound reporter 2 complex. Similar results are obtained for the d-methyl signals of reporter 3 in the free and bound state. The results are discussed and shown to be consistent with previous work utilizing absorption and circular dichroism techniques (see below). © 1969.