PMR STUDY OF THE COMPOSITION AND SEQUENCE OF URONATE RESIDUES IN ALGINATES

被引：425

作者：

GRASDALEN, H

LARSEN, B

SMIDSROD, O

机构：

[1] Institute of Marine Biochemistry, University of Trondheim

来源：

CARBOHYDRATE RESEARCH | 1979年 / 68卷 / 01期

关键词：

D O I：

10.1016/S0008-6215(00)84051-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequence and composition of uronate residues in intact alginate samples have been obtained by high-resolution 1H-n.m.r. spectroscopy. The viscosity problem was overcome by a slight, controlled depolymerization of the alginate samples before the 1H-n.m.r. spectra were recorded at 90°. The mannuronate (M)/guluronate (G) molar ratio was obtained from the intensities of the signals for the anomeric protons. A sequence-dependent deshielding of H-5 of the guluronate residues made it possible to determine the fractions of the four possible doublets of nearest neighbours along the chain. The results were wholly consistent with 13C-n.m.r. data. Significant deviation from comparable results obtained by chemical analysis appeared only for samples containing a large fraction of the mixed doublets. © 1979.

引用

页码：23 / 31

页数：9

共 15 条

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