PRESENCE OF A HUMAN CHORIONIC GONADOTROPIN-LIKE SUBSTANCE IN HUMAN-SPERM

被引:20
作者
ASCH, RH
FERNANDEZ, EO
SILERKHODR, TM
PAUERSTEIN, CJ
机构
[1] Department of Obstetrics and Gynecology, Center for Research and Training in Reproductive Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX
关键词
D O I
10.1016/0002-9378(79)90733-6
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
An hCG-like material has been extracted from human sperm. These experiments were designed to characterize this material. Sperms of 10 volunteers were separated from seminal fluid, washed in PBS three times, and resuspended in 0.5 ml of the same buffer. Samples were pooled; cells were disrupted by sonication and extracted in alkaline buffer by constant agitation at 4° C. The extract was ultracentrifuged at 4° C. Supernate was lyophilized and reconstituted in 2 cc of distilled water. This material presented a dose-response curve parallel to those of IS2-hCG and CR119 in βhCG RIA. When chromatographed in a Sephadex G-150 column the extract eluted within the hCG range and immunoreacted in the specific βhCG RIA. When absorbed onto a concanavalin A-Sepharose column, all recovered immunoreactive material eluted after exposure to α-d-methylglucoside, indicating that it is a glycoprotein. The extract stimulated progesterone and testosterone secretion in porcine granulosa cells and decapsulated rat testis, respectively, indicating its biologic potency. © 1979.
引用
收藏
页码:1041 / 1047
页数:7
相关论文
共 16 条