EFFECT OF DIFFERENT TARGET GASES ON LOW-ENERGY COLLISION-ACTIVATED DISSOCIATION OF PEPTIDES

被引：7

作者：

NAYLOR, S

LAMB, JH

机构：

[1] Mrc Toxicology Unit, Carshalton, Surrey, SM5 4EF, Woodmansterne Road

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1990年 / 4卷 / 07期

关键词：

D O I：

10.1002/rcm.1290040706

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Experimental variables affecting the daughter‐ion spectra of a series of protonated peptides [MH]+, produced by fast‐atom bombardment ionization, using a low energy (0–450 eV) quadruple collision cell are investigated. The parameters studied include target gas pressure, collision energy, cross‐sectional area and acidity of the target gas. The results show that low‐mass immonium ions are preferentially formed both at high collision energies (> 200 eV) and at target gas pressures > 10−6 mBar (where multiple collisions occur in the gas cell). Positive fragment ion abundance is maximized when acidic gases are used as the target gases, and this is rationalized on the basis of a proton‐transfer reaction from the target gas to the amide nitrogen of the peptide bond promoting fragmentation. Copyright © 1990 John Wiley & Sons Limited

引用

页码：251 / 255

页数：5

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