ISOLATION FROM RAT PERITONEAL LEUKOCYTES OF PLASMA-MEMBRANE ENRICHED IN ALKALINE-PHOSPHATASE AND A B-TYPE CYTOCHROME

被引：38

作者：

MILLARD, JA

GERARD, KW

SCHNEIDER, DL

机构：

[1] Department of Biochemistry, Dartmouth Medical School Hanover

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)91626-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A plasma membrane fraction more than 10-fold enriched in 5''-nucleotidase and alkaline phosphatase was prepared from peritoneal polymorphonuclear leukocytes. This fraction was highly enriched in MgATPase and a b-type cytochrome. High NADPH-duroquinone reductase activity was observed in the leukocytes, in addition to a lipid with quinone-like properties, neither of which cofractionated with plasma membrane. The possibility that an electron transport chain which functions to produce microbicidal O2 metabolites is subdivided between the plasma membrane and one of the cytoplasmic membranes in non-phagocytizing cells was proposed.

引用

页码：312 / 319

页数：8

共 28 条

[1] ANALYTICAL STUDY OF MICROSOMES AND ISOLATED SUBCELLULAR MEMBRANES FROM RAT-LIVER .4. BIOCHEMICAL, PHYSICAL, AND MORPHOLOGICAL MODIFICATIONS OF MICROSOMAL COMPONENTS INDUCED BY DIGITONIN, EDTA, AND PYROPHOSPHATE [J].