ISOLATION, EXPRESSION, AND NUCLEOTIDE SEQUENCING OF THE PILIN STRUCTURAL GENE OF THE BRAZILIAN PURPURIC FEVER CLONE OF HAEMOPHILUS-INFLUENZAE BIOGROUP AEGYPTIUS

被引：10

作者：

GEME, JWS

FALKOW, S

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL,ST LOUIS,MO 63110

[2] WASHINGTON UNIV,SCH MED,EDWARD MALLINCKRODT DEPT PEDIAT,ST LOUIS,MO 63110

[3] STANFORD UNIV,DEPT MICROBIOL & IMMUNOL,STANFORD,CA 94305

来源：

INFECTION AND IMMUNITY | 1993年 / 61卷 / 05期

关键词：

D O I：

10.1128/IAI.61.5.2233-2237.1993

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study we isolated the pilin gene from the Brazilian purpuric fever (BPF) clone of Haemophilus influenzae biogroup aegyptius, expressed the gene in Escherichia coli, and determined its nucleotide sequence. Comparison of the nucleotide sequence of the BPF pilin gene with the sequences of pilin genes from strains of H. influenzae sensu stricto demonstrated a high degree of identity. Consistent with this observation, hemagglutination inhibition studies performed with a series of glycoconjugates indicated that BPF pili and H. influenzae type b pili possess the same erythrocyte receptor specificity.

引用

收藏

页码：2233 / 2237

页数：5

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