PROTON-RESONANCE ASSIGNMENTS FOR PSEUDOMONAS-AERUGINOSA FERROCYTOCHROME-C-551

被引：10

作者：

CAI, M

TIMKOVICH, R

机构：

[1] Department of Chemistry University of Alabama, Tuscaloosa

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)91815-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A comparison between two sets of resonance assignments for ferrocytochrome c-551 from Pseudomonas aeruginosa reveals that major differences can be explained by pH effects. In turn, these reveal side chain protonation events in c-551 that markedly influence spectra. The behavior of resonances in a homologous protein from Pseudomonas stutzeri help to clarify ambiguities in the P. aeruginosa case. A corrected and completed set of proline assignments is presented. Labile side chain protons in residue 47. which hydrogen bonds to the inner heme propionate, appear to be in fast exchange with the solvent. © 1991.

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页码：309 / 314

页数：6

相关论文

共 12 条

[1] NMR COMPARISON OF PROKARYOTIC AND EUKARYOTIC CYTOCHROMES-C [J].

CHAU, MH ;

CAI, ML ;

TIMKOVICH, R .

BIOCHEMISTRY, 1990, 29 (21) :5076-5087

[2] SEQUENTIAL H-1-NMR ASSIGNMENTS OF IRON(II) CYTOCHROME-C551 FROM PSEUDOMONAS-AERUGINOSA [J].

DETLEFSEN, DJ ;

THANABAL, V ;

PECORARO, VL ;

WAGNER, G .

BIOCHEMISTRY, 1990, 29 (40) :9377-9386

[3] STRUCTURAL BASIS FOR THE VARIATION OF PH-DEPENDENT REDOX POTENTIALS OF PSEUDOMONAS CYTOCHROMES C-551 [J].

LEITCH, FA ;

MOORE, GR ;

PETTIGREW, GW .

BIOCHEMISTRY, 1984, 23 (08) :1831-1838

[4] STRUCTURE OF CYTOCHROME-C551 FROM PSEUDOMONAS-AERUGINOSA REFINED AT 1.6 A RESOLUTION AND COMPARISON OF THE 2 REDOX FORMS [J].

MATSUURA, Y ;

TAKANO, T ;

DICKERSON, RE .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 156 (02) :389-409

[5] NEW PERSPECTIVES ON C-TYPE CYTOCHROMES [J].

MEYER, TE ;

KAMEN, MD .

ADVANCES IN PROTEIN CHEMISTRY, 1982, 35 :105-212

[6]

Moore, 1987, CYTOCHROMES C BIOL A, P1, DOI 10.1007/978-3-642-72698-9_1

[7] STRUCTURAL BASIS FOR VARIATION IN REDOX POTENTIAL OF CYTOCHROMES [J].

MOORE, GR ;

WILLIAMS, RJP .

FEBS LETTERS, 1977, 79 (02) :229-232

[8] H-1-NMR STUDIES OF BACTERIAL CLASS-I CYTOCHROMES-C [J].

MOORE, GR .

JOURNAL OF INORGANIC BIOCHEMISTRY, 1986, 28 (2-3) :355-362

[9] PH-DEPENDENCE OF THE REDOX POTENTIAL OF PSEUDOMONAS-AERUGINOSA CYTOCHROME-C-551 [J].

MOORE, GR ;

PETTIGREW, GW ;

PITT, RC ;

WILLIAMS, RJP .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 590 (02) :261-271

[10] SPIN-ECHO WATER SUPPRESSION FOR THE GENERATION OF PURE-PHASE TWO-DIMENSIONAL NMR-SPECTRA [J].

SKLENAR, V ;

BAX, A .

JOURNAL OF MAGNETIC RESONANCE, 1987, 74 (03) :469-479