EFFECT OF LOW-PHOSPHATE DIET ON SODIUM/PHOSPHATE COTRANSPORT MESSENGER-RNA AND PROTEIN-CONTENT AND ON OOCYTE EXPRESSION OF PHOSPHATE-TRANSPORT

被引：19

作者：

BIBER, J ^{[1
]}

CADERAS, G ^{[1
]}

STANGE, G ^{[1
]}

WERNER, A ^{[1
]}

MURER, H ^{[1
]}

机构：

[1] UNIV ZURICH,DEPT PHYSIOL,CH-8057 ZURICH,SWITZERLAND

来源：

PEDIATRIC NEPHROLOGY | 1993年 / 7卷 / 06期

关键词：

PROXIMAL TUBULE; BRUSH BORDER MEMBRANE; PHOSPHATE DEPRIVATION; PROTEIN SYNTHESIS; EXPRESSION CLONING;

D O I：

10.1007/BF01213368

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

Recently, we have isolated a complementary DNA most likely related to rabbit kidney cortex brush border membrane sodium/phosphate (Na/P-i) cotransport activity [NaPi-1 (1)]. To further elucidate the cellular mechanisms involved in dietary 'adaptation' of renal Na/P-i cotransport, we have exposed young rabbits for 2 weeks to either a low phosphate (Pi) diet (LPD) or a high P-i diet (HPD). Initial linear uptake of Na/P-i cotransport in isolated brush border membrane Vesicles was increased in rabbits on a LPD compared with those on a HPD. Injection of equal amounts of total mRNA isolated from kidney cortex of LPD or HPD rabbits into Xenopus laevis oocytes resulted in a higher stimulation of Na-dependent oocyte P-i uptake in LPD than HPD preparations. No difference in the content of 'specific' mRNA (NaPi-1 cDNA probe, Northern blots) and of the content of the 'specific' brush border membrane protein (NaPi-1 antipeptide antibody, Western blots) between LPD and HPD preparations was observed. We conclude that 'chronic' dietary P-i deprivation leads to a protein synthesis-dependent alteration of Na/P-i cotransport activity which does not involve a change in the total amount of a protein related to the recently cloned NaPi-1 protein.

引用

页码：823 / 826

页数：4

共 22 条

[1]

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[2]

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[5]

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[7]

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[10]

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