EFFICIENT PROCESSING AND EXPORT OF HUMAN GROWTH-HORMONE BY HEAT-LABILE ENTEROTOXIN CHAIN-B SIGNAL SEQUENCE

被引：14

作者：

GHORPADE, A ^{[1
]}

GARG, LC ^{[1
]}

机构：

[1] NATL INST IMMUNOL, GENE REGULAT LAB, ARUNA ASAF ALI MARG, NEW DELHI 110067, INDIA

来源：

FEBS LETTERS | 1993年 / 330卷 / 01期

关键词：

SIGNAL SEQUENCE; PROCESSING; HUMAN GROWTH HORMONE; EXPORT;

D O I：

10.1016/0014-5793(93)80920-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The heat-labile enterotoxin chain B (LTB) signal sequence was used for the processing and export of human growth hormone (hGH). The protein was completely processed and exported across the cell membrane to accumulate in the periplasmic space in Escherichia coli. The human growth hormone cDNA was cloned as a PCR amplified fragment under the control of tac promoter and translationally fused to the LTB signal sequence. The rate of processing of hGH under the control of the LTB signal sequence was equal to or more than the rate of induction of expression, indicating efficient processing. The receptor binding activity of the processed periplasmic protein was established in a radio receptor assay.

引用

页码：61 / 65

页数：5

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