COQ AND SUCCINATE DEHYDROGENASE ACTIVITY OF KEILIN-HARTREE RESPIRATORY PARTICLE

被引：4

作者：

GAWRON, O

MAHAJAN, KP

LIMETTI, M

GLAID, AJ

机构：

[1] Department of Chemistry, Duquesne University, Pittsburgh

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 129卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-9861(69)90203-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Beef heart cytochrome-c deficient Keilin-Hartree, pH 5.7, particulate manifests decreased succinate-ferricyanide reductase activity after lyophilization and pentane extraction. Addition of CoQ10 or pentane extract restores activity in a Michaelis-Menten fashion, maximum activity being ≊ 5 × greater than that of extracted particulate. Kinetic relationships, 1 v vs. 1 (S) and 1 v vs. 1/(ferricyanide) plots, for extracted particulate are different from those of untreated particulate and soluble succinate dehydrogenase. Addition of CoQ10 restores the kinetic relationships to those seen with untreated particulate. It is concluded that CoQ plays an essential role in the succinate-ferricyanide reductase activity of the particulate. © 1969.

引用

页码：461 / &

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